The response factor f i `displaystyle f_`i` can be expressed on the basis of molar, volumic or mass. If the actual amount of sample and standard is the same: in chromatography, a response factor is defined as the ratio between the concentration of an analyzed compound and the sensor`s response to that connection. A chromatogram shows a sensor reaction as a peak. Although there are several ways to quantify the peak, one of the most common is the peak area, i.e. A indicates the signal (z.B. Peak Area) and the subscript i sample and subskripte st the standard.  Any factor is attributed to the standard response factor, for example. B 1 or 100. One of the main reasons for the use of response factors is the compensation for the inproductibility of manual injections in a gas phase chromatograph (GC). Injection volumes for GCs can be 1 microlitre or less and are difficult to reproduce. Differences in the volume of injected analyte lead to differences in peak areas in the chromatogram and all quantitative results are suspect. It is important to remember that variations in a gas phase chromatography (GC) system and an analysis method may cause a deviation from the response factor. In practice, a solution containing known amounts of octane and nonan is injected into a GC and a response factor, F, is calculated.
Then a separate solution is injected with an unknown amount of Antamund and a known amount of annonan. The response factor is applied to the data of the second solution and the unknown concentration of the octane is found. As the two compounds are in the same solution and are injected together, the terms volume are the same and break. The above equation is then reorganized to solve the k ratio. This report is then called the response factor, F. The F response factor corresponds to the constant reports of the k`s. Therefore, the F is constant. This means that, regardless of the amounts of octane and nonan, the ratio of the surface is always constant. The reaction factors calculated for each analyte are then used to determine the SRR between the two analytes: the response factor, usually in chromatography and spectroscopy, is the ratio between a signal generated by an analyte and the amount of analytes that produces the signal. Ideally and for a simple calculation, this ratio is the unit (one). In real-world scenarios, this is often not the case. Quantitative analysis is the determination of the concentration of a compound in a sample (analyt).
Reaction factors are important when GC is used for quantitative analysis. Therefore, reproducibility should be used to measure the sample, a method of eliminating the variability of the response factor. One of the simplest ways to eliminate changes in the response factor is to use relative response factors and an internal standard for GC calibration. Rome, K. – McIntyre, A. (2012). Intelligent use of relative reaction factors in the detection of flames in gas phase chromatography. Chromatography today, 52.
3) Make a sample of the unknown and a known concentration of IS and inject into the GC. After analysis, the required results are the advanced areas of A, B and IS. 2) Introduce the calibration sample into the GC. Because the concentrations of A, B and IS are known, the results can be used to calculate SRRS A and B in relation to IS: this example deals with the Analysis of Octane and Nonan, but can be applied to two arbitrary compounds. 4) A simple calculation calculates the concentration of A and B in the unknown. This conclusion is drawn because the ratio between the octane index and Nonan`s is lowest in mixture 1 and most in mixture 2.